Microcalorimetric Study on the Effect of Ce3+ on Halobacterium halobium R1

A microcalorimeric technique was used to evaluate the influence of rare earths Ce³⁺ on Halobacterium halobium R1 growth. By means of TAM air Thermal Activity Monitor, the thermogenic curves of H. halobium R1 growth were obtained. To analyze the results, the growth rate constant k and IC₅₀ were calculated, indicating that the values of k are linked to the concentration of Ce³⁺. The growth rate constant k of H. halobium R1 decreased gradually in the low concentration; thus, rare earths restrained the growth of H. halobium R1. On the contrary, as the concentration of Ce³⁺ became higher, the value of k for H. halobium R1 increased gradually, which showed Ce³⁺ stimulated the growth of H. halobium R1. When the concentration of rare earths became much higher, the value of k for H. halobium R1 also decreased, and the growth of H. halobium R1 was restrained totally in the end. By using transmission electron microscopy (TEM), it was observed that the transforming of H. halobium R1 in the different concentrations of Ce³⁺ confirmed the results derived from microcalorimetry. According to the thermogenic curves and TEM photos of H. halobium R1 under various conditions, it showed that there was some special effect about the interaction between rare earths and H. halobium R1 growth.