Purification of glyoxalase I from human erythrocytes by the use of affinity chromatography and separation of the three isoenzymes. |
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| Authors: | A C Aronsson G Tibbelin B Mannervik |
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| Institution: | Department of Biochemistry, Arrhenius Laboratory, University of Stockholm, S-106 91 Stockholm, Sweden |
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| Abstract: | Glyoxalase I has been purified to homogeneity from human erythrocytes. An essential part in the purification procedure involved affinity chromatography on S-hexylglutathione bound to epoxy-activated Sepharose 6B. Three isoenzymes were found, which could be separated by ion-exchange chromatography. Some of the properties of the enzyme are reported. |
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