A GFP fluorescence-based approach to determine detergent insolubility of the human serotonin1A receptor |
| |
Authors: | Kalipatnapu Shanti Chattopadhyay Amitabha |
| |
Institution: | Centre for Cellular and Molecular Biology, Uppal Road, Hyderabad 500 007, India. |
| |
Abstract: | Insolubility in non-ionic detergents such as Triton X-100 is a widely used biochemical criterion for characterization of membrane domains. We report here a novel green fluorescent protein fluorescence-based approach to directly determine detergent insolubility of specific membrane proteins. We have applied this method to explore the detergent resistance of an important G-protein coupled receptor, the serotonin1A (5-HT1A) receptor. Our results show, for the first time, that a small yet significant fraction of the 5-HT1A receptor exhibits detergent insolubility. These results are validated by control experiments involving fluorescent lipid probes and protein markers. Our results assume relevance in the context of localization of the 5-HT1A receptor in membrane domains and its significance in receptor function and signaling. |
| |
Keywords: | Detergent insolubility Enhanced yellow fluorescent protein fluorescence DiI Membrane domain Triton X-100 Serotonin1A receptor |
本文献已被 ScienceDirect PubMed 等数据库收录! |