影响沙田柚叶片离体培养的因素研究

研究了离体培养条件下影响沙田柚叶片愈伤组织诱导和分化的一些因素。结果表明 ,2 ,4 D可以诱导愈伤组织的形成 ,高浓度的蔗糖 (6% )显著提高叶片愈伤诱导率与愈伤组织重量 ,且在 2 ,4 D和蔗糖之间存在着相互作用。外源GA3处理抑制愈伤组织的诱导和生长 ,而CCC与ABA处理显著提高叶片的愈伤诱导率和愈伤组织生长量。愈伤组织转移到附加 3 .0mg/LBA的MS分化培养基上可以分化出芽 ,0 .2 5mg/LGA3的加入可以进一步提高愈伤组织的分化率和每块愈伤组织的再生芽数。

Factors affecting in vitro shoot regeneration from leaf explants of Citrus grandis(L.) Osbeck cv.Shatian-Yu

Some factors were investigated in the shoot regeneration from the leaf explants of Citrus grandis (L.) Osbeck cv. Shatian-Yu. The results showed that 2,4-D could induce the callus formation from leaf explants. The addition of the sucrose at 6% significantly increase the callusing frequency and the callus growth. The interaction existed between 2,4-D and the sucrose. Exogenous GA3 inhibited the callus formation and their growth. Chlorocholine chloride (CCC) and abscisic acid (ABA) treatments, however, significantly increased callusing frequency and the callus growth. Adventitious shoots were differentiated when calli were transferred to MS medium supplemented with 3.0mg/L BA. The addition of GA3 at 0.25 mg.L~(-1) greatly increased regeneration frequency and the regenerated shoots number per callus.