Production and partial purification of membrane proteins using a liposome-supplemented wheat cell-free translation system |
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| Authors: | Akira Nozawa Tomio Ogasawara Satoko Matsunaga Takahiro Iwasaki Tatsuya Sawasaki Yaeta Endo |
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| Institution: | (1) Cell-Free Science and Technology Research Center and the Venture Business, Laboratory, Ehime University, 3 Bunkyo-Cho, Matsuyama Ehime, 790-8577, Japan;(2) RIKEN Systems and Structural Biology Center, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama Kanagawa, 230-0045, Japan;(3) Proteo-Medicine Research Center, Ehime University, Toon Ehime, 791-0295, Japan |
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| Abstract: | Background Recently, some groups have reported on cell-free synthesis of functional membrane proteins (MPs) in the presence of exogenous
liposomes (liposomes). Previously, we reported synthesis of a functional AtPPT1 plant phosphate transporter that was associated
with liposomes during translation. However, it is unclear whether or not lipid/MP complex formation is common to all types
of MPs in the wheat cell-free system. |
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