|
|||||
|
|
Expression of microRNA-1, microRNA-133a and Hand2 protein in cultured embryonic rat cardiomyocytes |
|
| Authors: | Zhonggang Feng Rie Takahashi Takao Nakamura Daisuke Sato Nobuyuki Shirasawa Ayako Nakayama Sakura Kurashige Tadashi Kosawada Tatsuo Kitajima Mitsuo Umezu |
| Institution: | 1. Graduate School of Science and Engineering, Yamagata University, Yonezawa-shi, Japan 2. Graduate School of Medical Science, Yamagata University, Yamagata, Japan 3. Department of Anatomy and Structural Science, Yamagata University, Yomagata, Japan 4. Electronic Systems Engineering, Malaysia-Japan International Institute of Technology, Kuala Lumpur, Malaysia 5. Integrative Bioscience and Biomedical Engineering, Graduate School of Waseda University, Tokyo, Japan |
| Abstract: | In this study, we investigated the expression of the pathway, SRF–microRNA-1/microRNA-133a–Hand2, in the Wistar rat embryonic ventricular cardiomyocytes under conventional monolayer culture. The morphological observation of the cultured cardiomyocytes and the mRNA expression levels of three vital constituent proteins, MLC-2v, N-cadherin, and connexin43, demonstrated the immaturity of these cultured cells, which was featured by less myofibril density, immature sarcomeric structure, and significantly lower mRNA expression of the three constituent proteins than those in neonatal ventricular samples. More importantly, results in this study suggest that the change of SRF–microRNA-1/microRNA-133a–Hand2 pathway results into the attenuation of the Hand2 repression in cultured cardiomyocytes. These outcomes are valuable to understand the cellular state as embryonic cardiomyocytes to be in vitro model and might be useful for the assessment of engineered cardiac tissue and cardiac differentiation of stem cells. |
| Keywords: | |
| 本文献已被 SpringerLink 等数据库收录！ | |