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Translocation of cAMP-dependent protein kinase to the nucleus during development of Dictyostelium discoideum
Authors:C Woffendin  T C Chambers  K L Schaller  B H Leichtling  H V Rickenberg
Affiliation:1. Department of Molecular and Cellular Biology, National Jewish Center for Immunology and Respiratory Medicine, Denver, Colorado 80262 USA;2. Department of Biochemistry, Biophysics and Genetics, University of Colorado Health Sciences Center, Denver, Colorado 80262 USA;1. School of Life Sciences and Biotechnology, The Joint International Research Laboratory of Metabolic & Developmental Sciences, Shanghai Jiao Tong University, Shanghai 200240, China;2. Shanghai Collaborative Innovation Center of Agri-Seeds, Joint Center for Single Cell Biology, Shanghai 200240, China;3. School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, China;4. Zhiyuan College, Shanghai Jiao Tong University, Shanghai 200240, China;1. Division of Genetics and Genomics, Boston Children’s Hospital, Boston, MA, USA;2. Departments of Pediatrics and Neurology, Harvard Medical School, Boston, MA, USA;3. Allen Discovery Center for Human Brain Evolution, Boston Children’s Hospital and Harvard Medical School, Boston, MA, USA;4. Howard Hughes Medical Institute, Chevy Chase, Maryland, USA;1. Department of Biology, University of Pennsylvania, Philadelphia, PA 19104, USA;2. School of Biological Sciences, University of Bristol, Bristol BS8 1TQ, UK;1. Department of Genetics, Perelman School of Medicine, Philadelphia, PA, USA;2. Department of Pediatrics, University of Washington, Seattle, WA, USA;3. Seattle Children’s Research Institute, University of Washington, Seattle, WA, USA;1. Key Laboratory of Urban Agriculture (South) Ministry of Agriculture and School of Agriculture and Biology, Shanghai Jiaotong University, Shanghai 200240, China;2. National Key Laboratory of Plant Molecular Genetics (NKLPMG), Institute of Plant Physiology and Ecology (SIPPE), Shanghai Institutes for Biological Sciences (SIBS), Shanghai 200032, China;3. School of Life Sciences and Biotechnology, Shanghai Jiaotong University, Shanghai 200240, China;1. Department of Biology, Washington University in St. Louis, St. Louis, MO 63130, USA;2. Department of Environmental and Plant Biology, Ohio University, Athens, OH 45701, USA
Abstract:The distribution of the catalytic and regulatory subunits of the cAMP-dependent protein kinase between cytoplasm and nucleus was determined during the development of Dictyostelium discoideum. In vegetative amoebae approximately 2% of the subunits were in the nucleus. During development there was an approximately 5-fold increase in total soluble cAMP-dependent protein kinase and a 15- to 30-fold increase of enzyme in the nuclear fraction. There was a reverse translocation from nucleus to cytoplasm, when Tipped Aggregates were disrupted and the resultant amoebae incubated in single-cell suspension. The addition of cAMP to these single-cell suspensions brought about the reentry of the subunits into the nucleus. The findings are discussed in relation to the potential role of the cAMP-dependent protein kinase in the regulation of mRNA and protein synthesis.
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