An enzymatic assay for DNA in epoxy-tissue-sections by light microscope-autoradiography |
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| Authors: | S P Modak G M Donnelly S Karasaki C V Harding J R Reddan N Unakar |
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| Affiliation: | 1. Department of Molecular Biology, ISREC, Bugnon 21, 1011 Lausanne, Switzerland;2. Department of Cell Biology, Medical Center, University of Kentucky, Lexington, Ky 40506, USA;3. Institut du Cancer de Montréal, Laboratoire de Recherches, Hôpital Notre-Dame, Montréal 133, Canada;4. Department of Biological Sciences, Oakland University, Rochester, Mich. 48063, USA |
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| Abstract: | Thick and thin sections of glutaraldehyde- and osmium-fixed and epoxy-embedded tissues were incubated with calf-thymus terminal deoxynucleotidyl transferase, and the resulting incorporation of 3H-dAMP was located by light microscope-autoradiography. This cyto-enzymatic method is rapid and permits detection of nuclear DNA in a large variety of cell types from the rat trachea, rabbit lens epithelium, and newt ovaries. |
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