Identification of Gibberellins in Norway Spruce (Picea abies [L.] Karst.) by Combined Gas Chromatography-Mass Spectrometry |
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| Authors: | Odén P C Schwenen L Graebe J E |
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| Institution: | Department of Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences, S-901 83 Umeå, Sweden;Pflanzenphysiologisches Institut der Universität, Untere Karspüle 2, D-3400 Göttingen, Federal Republic of Germany |
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| Abstract: | Gibberellins A1 (GA1), A3 and A9 were identified from extracts of shoots of 6-month old Norway spruce (Picea abies) seedlings by the use of sequential reverse and normal phase high performance liquid chromatography (HPLC), bioassay, radioimmunoassay (RIA) and combined gas chromatography-mass spectrometry (GC-MS). The bioassay and RIA were used after fractionation by HPLC to detect the GA-containing fractions, which were then examined by GC-MS. The GAs identified are considered to be endogenous. |
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