Mouse FKBP23 mediates conformer-specific functions of BiP by catalyzing Pro117 cis/trans isomerization |
| |
| Authors: | Feng Miao Gu Chao Ma Shikui Wang Ying Liu Huijuan Han Ruifang Gao Junfei Long Yi Mi Huaifeng |
| |
| Institution: | aDepartment of Veterinary Preventive Medicine, College of Animal Science and Veterinary Medicine, Jilin University, Xi’an Road 5333, Changchun, Jilin 130062, China;bLehrstuhl für Experimentelle Genetik, Technische Universität München, Freising-Weihenstephan 85354, Germany;cComprehensive Pneumology Center, Institute of Lung Biology and Disease, Helmholtz Zentrum München, Ingolstädter Landstraße 1, D-85764 Munich, Germany;dInstitute of Experimental Genetics, Helmholtz Zentrum München, Ingolstädter Landstraße 1, D-85764 Munich, Germany;eCollege of Quartermaster Technology, Jilin University, Xi’an Road 5333, Changchun, Jilin 130062, China;fDepartment of Food Science, College of Agriculture, Yanbian University, Gongyuan 977, Yanji, Jilin 133002, China;gInstitute of Animal Husbandry and Veterinary Science of Jilin, Xi’an Road 4510, Changchun, Jilin 130062, China |
| |
| Abstract: | Gene expression analysis is frequently used to analyze the response to viral infection, and 18S RNA, SHDA and GAPDH represent popular house keeping genes (HKGs) often used to normalize gene expression. Here we describe the first systematic selection and evaluation of suitable HKGs for gene expression analysis in chicken embryo fibroblasts (CEF) infected with NDV adapted to the guidelines from Gorzelniak and Ferguson. Our results indicate that ACTB, HPRT1 and HMBS were valuable and stable HKGs, while 18S RNA, GAPDH and SHDA are considerably regulated during the course of infection and thus precluded for normalization. Normalizing the infection dependent gene IFN-a and the infection independent gene B2M to inappropriate HKGs consequently misleads to significant errors in estimating their regulations. Our study emphasizes that even the most popular HKGs like 18S RNA and GAPDH can lead to divergent and inaccurate data interpretation of significant magnitude if not carefully analyzed for stability before. |
| |
| Keywords: | Newcastle disease virus Housekeeping gene qRT-PCR Chicken embryo fibroblasts |
| 本文献已被 ScienceDirect PubMed 等数据库收录！ |
|
