CTGF/CCN2 has a chemoattractive function but a weak adhesive property to embryonic carcinoma cells |
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| Authors: | Aguiar Diego P Pontes Bruno Mendes Fabio A Andrade Leonardo R Viana Nathan B Abreu José G |
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| Affiliation: | aLaboratório de Embriologia de Vertebrados, Instituto de Ciências Biomédicas, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Brazil;bLPO-COPEA, Instituto de Ciências Biomédicas, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Brazil;cLaboratório de Biomineralização, Instituto de Ciências Biomédicas, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Brazil;dInstituto de Física, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Brazil |
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| Abstract: | Connective tissue growth factor (CTGF/CCN2) is a protein of the CCN family that modulates cell–ECM interactions in a variety of cell types. In this study, we investigated the chemotactic and adhesive properties of CCN2 protein in embryonic teratocarcinoma P19 cells. Initially, P19 cells were attracted to CCN2-coated agarose beads. In Boyden chamber experiments, CCN2-containing medium induced a threefold greater migration of P19 cells. CCN2 adhesion properties were studied by using optical tweezers. The specific adhesion times of P19 cells to polystyrene beads coated with laminin, fibronectin, CCN2 and bovine serum albumin were 1.8 ± 0.5s, 2.7 ± 0.4s, 10 ± 2s and 13 ± 2s, respectively, revealing an unexpectedly low adhesive capacity of CCN2 protein for P19 cells. In conclusion, our findings support the chemoattractive role of CCN2 for P19 cells, but not its adhesive role when compared to laminin or fibronectin. |
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| Keywords: | Adhesion CCN2 Chemoattraction Migration Optical tweezers P19 cells |
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