Oxidized low-density lipoprotein-induced periodontal inflammation is associated with the up-regulation of cyclooxygenase-2 and microsomal prostaglandin synthase 1 in human gingival epithelial cells |
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Authors: | Nagahama Yu Obama Takashi Usui Michihiko Kanazawa Yukari Iwamoto Sanju Suzuki Kazushige Miyazaki Akira Yamaguchi Tomohiro Yamamoto Matsuo Itabe Hiroyuki |
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Institution: | aDepartment of Periodontology, School of Dentistry, Showa University Dental Hospital, Tokyo, Japan;bDepartment of Biological Chemistry, Showa University School of Pharmacy, Tokyo, Japan;cDepartment of Biochemistry, Showa University School of Medicine, Tokyo, Japan |
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Abstract: | Periodontitis is characterized by chronic gingival tissue inflammation, and inflammatory mediators such as IL-8 and prostaglandin E2 (PGE2) are associated with disease progression. Previously we showed that oxidatively modified low-density lipoprotein (oxLDL) was present in gingival crevicular fluid. In this study, the role of oxLDL in the gingival epithelial cell inflammatory response was further investigated using Ca9-22 cells and primary human oral keratinocytes (HOK). Treatment of Ca9-22 cells and HOK with oxLDL induced an up-regulation of IL-8 and the PGE2-producing enzymes, cyclooxygenase-2 and microsomal PGE2 synthase-1. These responses induced by oxLDL were significantly suppressed by a nuclear factor-kappa B (NF-κB) inhibitor. However, unlike the result in macrophages, oxLDL did not lead to an increase in CD36 expression in these two cells. These results suggest that oxLDL elicits gingival epithelial cell inflammatory responses through an activation of the NF-κB pathway. These data suggest a mechanistic link between periodontal disease and lipid metabolism-related disorders, including atherosclerosis. |
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Keywords: | Abbreviations: Abs antibodys CHD coronary heart disease COX cyclooxygenase FBS fetal bovine serum GCF gingival crevicular fluid HOK human oral keratinocyte IL-8 interleukin-8 LDL low-density lipoprotein mPGES microsomal prostaglandin E synthase oxLDL oxidized low-density lipoprotein PBS phosphate-buffered saline PC phosphatidylcholine PGE2 prostaglandin E2 |
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