Comparing mechano-transduction in fibroblasts deficient of focal adhesion proteins |
| |
Authors: | Dey Tuli Mann Melanie C Goldmann Wolfgang H |
| |
Affiliation: | Department of Physics, Friedrich-Alexander-University of Erlangen-Nuremberg, Erlangen, Germany |
| |
Abstract: | Mechano-transduction was studied in wildtype and focal adhesion (FA) protein-deficient mouse embryonic fibroblasts (MEFs). Using a cell stretcher, we determined the effect of stretch on cell morphology, apoptosis, and phosphorylation of ERK1/2. After 20% cyclic, uniaxial stretch, FA-deficient MEFs showed morphological changes and levels of apoptosis of the order: focal adhesion kinase > p130Cas > vinculin compared to wildtype cells. ERK1/2 phosphorylation peaked in wildtype cells at around 10 min, and in all FA-deficient cells at around 5 min. The relative change in strain energy of FA-deficient cells compared to wildtype cells was of the order: vinculin > FAK > p130Cas. Taken together, FAK and p130Cas are more important in the stretch-mediated downstream signaling and cell survival pathway, while vinculin is more critical in maintaining cell contractility. |
| |
Keywords: | Mouse embryonic fibroblasts Focal adhesions Focal adhesion kinase (FAK) Vinculin p130CAS Actin cytoskeleton Cell stretcher Cell viability Cell tractions |
本文献已被 ScienceDirect PubMed 等数据库收录! |
|