Production of poly-β-hydroxybutyrate by Azotobacter chroococcum H23 in chemically defined medium and alpechin medium |
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Authors: | MV Martinez-Toledo J Gonzalez-Lopez B Rodelas C Pozo V Salmeron |
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Institution: | Group of Environmental Microbiology, Department of Microbiology, Faculty of Pharmacy and Institute of Water Research, University of Granada, Granada, Spain |
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Abstract: | M.V. MARTINEZ-TOLEDO, J. GONZALEZ-LOPEZ, B. RODELAS, C. POZO AND V. SALMERON. 1995. Azotobacter chroococcum H23 is able to produce large amounts of poly-β-hydroxybutyrate (PHB) during growth in chemically-defined medium (N-free or with NH+4) and alpechin (wastewater from olive oil mills) medium. Polymer production was not dependent of the nutrient limitation. Strain H23 was capable of accumulating PHB up to 70% of the cell dry weight after 24 h incubation in chemically-defined media containing 1% glucose, fructose, mannitol, saccharose or starch. Azotobacter chroococcum grown on NH+4-medium supplemented with alpechin formed PHB up to 50% of the cell dry weight after 24 h, suggesting that these wastes could be utilized by Azotobacter as a cheap substrate for producing PHB. |
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