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A marine bacterium accumulates polyhydroxyalkanoate consisting of mainly 3-hydroxydodecanoate and 3-hydroxydecanoate
Authors:Wang  Qian  Zhang  Hanxing  Chen  Quan  Chen  Xiulan  Zhang  Yuzhong  Qi  Qingsheng
Affiliation:(1) State Key Laboratory of Microbial Technology, Life Science School, Shandong University, 250100 Jinan, People’s Republic of China;
Abstract:A deep-sea psychrotrophic bacterium Pseudoaltermonas sp. SM9913, isolated from abyssalbenthic sediments in Bohai Sea, was found to be able to synthesize polyhydroxyalkanoate (PHA) polymer consisting of mainly 3-hydroxydecanoate and 3-hydroxydodecanoate. PHA accumulation in Pseudoaltermonas sp. SM9913 was confirmed by transmission electron microscope, Nile red dye and gas chromatography/mass spectral. The PHA content was determined as high as 2–3% of cell dry weight when the strain was grown in a sea water-based liquid medium. The relationship between PHA and exopolysaccharide accumulation in this strain was discussed.
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