Effect of bacterial inoculation,plant genotype and developmental stage on root-associated and endophytic bacterial communities in potato (<Emphasis Type="Italic">Solanum tuberosum</Emphasis>) |
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Authors: | Fernando Dini Andreote Ulisses Nunes da Rocha Welington Luiz Araújo João Lúcio Azevedo Leonard Simon van Overbeek |
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Institution: | 1.Departamento de Ciência do Solo,Escola Superior de Agricultura “Luiz de Queiroz”, ESALQ/USP,Piracicaba,Brazil;2.Plant Research International,Wageningen,The Netherlands;3.Laboratory of Microbial Ecology and Molecular Biology,University of Mogi das Cruzes,Mogi das Cruzes,Brazil;4.Department of Genetics,Escola Superior de Agricultura “Luiz de Queiroz”, University of S?o Paulo,Piracicaba,Brazil |
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Abstract: | Beneficial bacteria interact with plants by colonizing the rhizosphere and roots followed by further spread through the inner
tissues, resulting in endophytic colonization. The major factors contributing to these interactions are not always well understood
for most bacterial and plant species. It is believed that specific bacterial functions are required for plant colonization,
but also from the plant side specific features are needed, such as plant genotype (cultivar) and developmental stage. Via
multivariate analysis we present a quantification of the roles of these components on the composition of root-associated and
endophytic bacterial communities in potato plants, by weighing the effects of bacterial inoculation, plant genotype and developmental
stage. Spontaneous rifampicin resistant mutants of two bacterial endophytes, Paenibacillus sp. strain E119 and Methylobacterium mesophilicum strain SR1.6/6, were introduced into potato plants of three different cultivars (Eersteling, Robijn and Karnico). Densities
of both strains in, or attached to potato plants were measured by selective plating, while the effects of bacterial inoculation,
plant genotype and developmental stage on the composition of bacterial, Alphaproteobacterial and Paenibacillus species were determined by PCR-denaturing gradient gel-electrophoresis (DGGE). Multivariate analyses revealed that the composition
of bacterial communities was mainly driven by cultivar type and plant developmental stage, while Alphaproteobacterial and
Paenibacillus communities were mainly influenced by bacterial inoculation. These results are important for better understanding the effects
of bacterial inoculations to plants and their possible effects on the indigenous bacterial communities in relation with other
plant factors such as genotype and growth stage. |
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