Optimization of lipase-catalyzed glucose ester synthesis in ionic liquids |
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Authors: | Sung Ho Ha Nguyen Minh Hiep Sang Hyun Lee Yoon-Mo Koo |
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Institution: | (1) ERC for Advanced Bioseparation Technology, Inha University, Incheon, 402-751, Korea;(2) Department of Biological Engineering, Inha University, Incheon, 402-751, Korea;(3) Department of Microbial Engineering, Konkuk University, Seoul, 143-701, Korea |
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Abstract: | Lipase-catalyzed esterification of glucose with fatty acids in ionic liquids (ILs) mixture was investigated by using supersaturated
glucose solution. The effect of ILs mixture ratio, substrate ratio, lipase content, and temperature on the activity and stability
of lipase was also studied. The highest yield of sugar ester was obtained in a mixture of 1-butyl-3-methylimidazolium trifluoromethanesulfonate
(Bmim]TfO]) and 1-methyl-3-octylimidazolium bis(trifluoromethyl)-sulfonyl]amide (Omim]Tf2N]) with a volume ratio of 9:1, while Novozym 435 (Candida antarctica type B lipase immobilized on acrylic resin) showed the optimal stability and activity in a mixture of Bmim]TfO] and Omim]Tf2N] with a 1:1 volume ratio. Reuse of lipase and ILs was successfully carried out at the optimized reaction conditions. After
5 times reuse of Novozym 435 and ILs, 78% of initial activity was remained. |
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