Microspectrographic analysis of formaldehyde-induced fluorescence in the quail adenohypophysis after injection of l-dopa and 5-hydroxytryptophan |
| |
Authors: | F. Harrisson M. Liebens L. Vakaet J. M. Lauweryns |
| |
Affiliation: | (1) Department of Anatomy and Embryology, State University of Antwerp, 171 Groenenborgerlaan, B-2020 Antwerpen, Belgium;(2) Department of Pulmonary Histopathology, Catholic University of Leuven, 12 Minderbroederstraat, B-3000 Leuven, Belgium |
| |
Abstract: | Summary The spectral distribution of the formaldehyde-induced fluorescence emitted by model solutions and by adenohypophyses after intraperitoneal injection of l-dopa or 5-hydroxytryptophan was analyzed microspectrographically. Based on previously reported studies and on present findings, it seems that dopamine is stored in the strongly fluorescent cells after injection of l-dopa, and that a compound closely related to 5-hydroxytryptophan or serotonin is present in most of the cells after injection of 5-hydroxytryptophan. A non-specific, granular fluorescence appeared after 5-hydroxytryptophan and, to a lesser extend, l-dopa treatment. It probably represents autofluorescence of lysosomes, which are numerous in these circumstances. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|