Isolation of the amyloid P component from the Engelbreth-Holm-Swarm (EHS) tumor of the mouse |
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Authors: | S Inoue M Skinner C P Leblond T Shirahama A S Cohen |
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Affiliation: | 1. Department of Anatomy, McGill University, Montreal, Quebec, Canada H3A 2B2 |
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Abstract: | The amyloid P component was isolated from the mouse EHS tumor, a producer of basement membrane-like material. Following collagenase treatment of the tissue homogenate and centrifugation, the supernatant was purified by calcium-dependent binding to agarose, and elution with ethylenediaminetetraacetic acid. Identification of the purified material as the amyloid P component was established by immunodiffusion and electron microscopic appearance as 8.5 nm pentagonal units, frequently assembled into columns. SDS-PAGE gel electrophoresis yielded 25,000 D bands, suggesting that the amyloid P is of the mouse type. It is proposed that the mouse amyloid P component extracted from the tumor is located within the basotubules present in the pericellular matrix. |
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