Chromosome reshuffling in birds of prey: the karyotype of the world's largest eagle (Harpy eagle, Harpia harpyja) compared to that of the chicken (Gallus gallus) |
| |
Authors: | Edivaldo H C de Oliveira Felix A Habermann Oneida Lacerda Ives J Sbalqueiro Johannes Wienberg Stefan Müller |
| |
Institution: | (1) Laboratório de Citogenética, Depto de Genética, Universidade Federal do Pará, Belém, PA, Brazil;(2) Chair of Animal Breeding, Technical University of Munich, Munich, Germany;(3) Depto de Zoológicos, Prefeitura Municipal de Curitiba, Curitiba, PR, Brazil;(4) Laboratório de Citogenética Animal, Depto de Genética, Universidade Federal do Paraná, Curitiba, PR, Brazil;(5) Department Biology II, Human Genetics, Ludwig Maximilians University, Groβhaderner Strasse 2, 82152 Planegg-Martinsried, Germany;(6) Institute of Human Genetics, GSF National Research Center for Environment and Health, Munich, Germany |
| |
Abstract: | Like various other diurnal birds of prey, the world's largest eagle, the Harpy (Harpia harpyja), presents an atypical bird karyotype with 2n=58 chromosomes. There is little knowledge about the dramatic changes in the genomic reorganization of these species compared
to other birds. Since recently, the chicken provides a “default map” for various birds including the first genomic DNA sequence
of a bird species. Obviously, the gross division of the chicken genome into relatively gene-poor macrochromosomes and predominantly
gene-rich microchromosomes has been conserved for more than 150 million years in most bird species. Here, we present classical
features of the Harpy eagle karyotype but also chromosomal homologies between H. harpyja and the chicken by chromosome painting and comparison to the chicken genome map. We used two different sets of painting probes:
(1) chicken chromosomes were divided into three size categories: (a) macrochromosomes 1–5 and Z, (b) medium-sized chromosomes
6–10, and (c) 19 microchromosomes; (2) combinatorially labeled chicken chromosome paints 1–6 and Z. Both probe sets were visualized
on H. harpyja chromosomes by multicolor fluorescence in situ hybridization (FISH). Our data show how the organization into micro- and macrochromosomes
has been lost in the Harpy eagle, seemingly without any preference or constraints. |
| |
Keywords: | |
本文献已被 PubMed SpringerLink 等数据库收录! |
|