Bacteriophage lambda-E. coli K12 vector-host system for gene cloning and expression under lactose promoter control: I. DNA fragment insertion at the lacZ EcoRI restriction site. |
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Authors: | Christine Pourcel Christian Marchal Anne Louise Alexandre Fritsch Pierre Tiollais |
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Affiliation: | (1) Recombinaison et expression génétique (INSERM U. 163), Unité de Génie Génétique, Institut Pasteur, 28, rue du Docteur Roux, F-75015 Paris, (France) |
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Abstract: | Summary Bacteriophage lambda vectors, derived from plac5 were constructed. Their genomes have only one EcoRI restriction site, located near the end of the -galactosidase gene. Recombinants, constructed in vitro, having a DNA fragment inserted in the EcoRI site, are lac- and can be easily recognized. Expression of such foreign genes is then under the control of the lac promoter. Mutations Qam73 and Sam7 greatly increase the amount of -galactosidase synthesized by the vector bacteriophage. The ZEQS vector has been certified B2 (EK2) by the French control commission Recombinaisons génétiques in vitro. |
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