T-DNA as a gene tag |
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Authors: | Richard W Hayashi H Schell J |
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Institution: | Max-Planck-institut für Züchtungsforschung, Carl von Linne Weg 10, D5000 K?ln 30, Germany. |
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Abstract: | The characteristics of Agrobacterium-mediated plant transformation are such that the T-DNA can be used as an insertional mutagen and, by extension, a gene tag. An increasing number of mutants have been obtained as a result of T-DNA insertion and the versatility of this experimental system can be exploited further to tag sequences of DNA which control gene expression. While the isolation of specific mutations by T-DNA tagging in the past has been fortuitous, T-DNA-based vectors have the potential to generate transgenic plants with specific, selectable mutations. |
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