Characterization of the Ca2+-regulatory complex of chick embryonic muscles: Polymorphism of tropomyosin in adult and embryonic fibers |
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Authors: | Raman K Roy James D Potter Satyapriya Sarkar |
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Institution: | 1. Department of Muscle Research, Boston Biomedical Research Institute, Boston, Mass., 02114 USA;2. Department of Neurology, Harvard Medical School, Boston, Mass., 02114 USA |
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Abstract: | The Ca2+-regulatory tropomyosin-troponin complex was purified from chick embryonic muscles by a combination of DEAE-cellulose chromatography and (NH4)2SO4 fractionation. The embryonic complex was very similar to that obtained from adult chicken muscles with respect to stoichiometry of components and biological activity. Tropomyosin of embryonic skeletal muscles contains both α and β subunits, the β form being the major species. In the adult stage the β form is decreased with a concomitant increase in the α form. These results indicate that i) the Ca2+-regulatory proteins are not deficient in early embryonic muscles as previously thought (Hitchcock, S.E., Develop. Biol. , 399, 1970), and ii) different structural genes coding for tropomyosin subunits are expressed differentially in embryonic and adult muscle fibers. |
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Keywords: | Tn troponin TnT troponin T TnI troponin I TnC troponin C TM tropomyosin TM-Tn tropomyosin-troponin Tm single polypeptide chain of tropomyosin AM actomyosin DTT dithiothreitol SDS sodium dodecyl sulfate EGTA ethylene glycol bis (β-aminoethylether)-N N-tetraacetate |
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