mutants deficient in exoribonucleases |
| |
Authors: | Nikolai Nikolaev Virginia Folsom David Schlessinger |
| |
Affiliation: | Department of Microbiology and Immunology, Division of Biology and Biomedical Sciences, Washington University, St. Louis, Mo. 63110 USA. |
| |
Abstract: | Strain S296, isolated by screening 2000 colonies after nitrosoguanidine mutagenesis, yields extracts with less than 1% of wild-type RNase activity against (3H) poly(U). Unlike other strains, S296 grows with a doubling time of about 2 hr., both in nutrient broth and in minimal medium, and at 30°, 37° and 42°. The strain retains 10 to 20% of wild-type exonuclease activity against (3H) rRNA or T4 phage-specific mRNA; but two further mutants, made by screening mutagenized colonies of strain S296, are reduced to 3% of wild-type activity against those substrates as well. |
| |
Keywords: | |
本文献已被 ScienceDirect 等数据库收录! |
|