Fluorometric assay of sialic acid in the picomole range: A modification of the thiobarbituric acid assay |
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Authors: | K.S. Hammond D.S. Papermaster |
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Affiliation: | Department of Pathology, Yale Medical School, New Haven, Connecticut 06510 USA |
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Abstract: | Fluorescence of the thiobarbituric acid reaction product of periodate-cleaved sialic acid is readily detected by excitation at 550 nm and analysis of emitted light at 570 nm. A fluorometric microassay is described which is 500-fold more sensitive than the usual spectrophotometric procedures and readily detects 10 ng (32 pmol) of sialic acid. Contamination by 2-deoxyribose from cellular material is easily detected by shifts of excitation maxima below 550 nm. |
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