Ethanol-induced cytochrome P-450: Catalytic activity after partial purification |
| |
| Authors: | J-P Villeneuve P Mavier J-G Joly |
| |
| Institution: | 1. Clinical Research Center, Hôpital Saint-Luc, Montréal, Canada.;2. Université de Montréal, Montréal, Canada. |
| |
| Abstract: | Cytochrome P-450 was partially purified from liver microsomes obtained from control, ethanol, phenobarbital, and 3-methylcholanthrene-treated rats. Benzphetamine demethylation, benzpyrene hydroxylation and aniline hydroxylation activities were assayed in a reconstituted system using fixed amounts of reductase and lipids, and increasing amounts of cytochrome P-450 from each source. Cytochrome P-450 from ethanol-fed rats showed substrate specificity differing from cytochrome P-450 obtained from control, phenobarbital and 3-methylcholanthrene-treated rats. |
| |
| Keywords: | Request for reprints should be addressed to: Jean-Gil Joly M D Hôpital Saint-Luc 1058 St-Denis Montréal P Q H2X 3J4 Canada |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
