Collagen fractionation: Separation of native types I,II and III by differential prectipitation |
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| Authors: | Robert L Trelstad Veronica M Catanese Debra F Rubin |
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| Institution: | 1. Developmental Biology Laboratory, Department of Medicine, Harvard Medical School, Massachusetts General Hospital, Boston, Massachusetts 02114 USA;2. Department of Pathology, Harvard Medical School, Massachusetts General Hospital, Boston, Massachusetts 02114 USA |
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| Abstract: | Collagen types I, II and III can be purified in their native state from heterogeneous collagen solutions by fractional precipitation at neutral pH using ammonium sulfate, sodium chloride and ethanol as precipitants. This method of collagen separation is useful as a preparative procedure and should also serve as an analytical tool for identification of unknown radioactively labeled collagens. |
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