Electrophoretic behavior of oligonucleotides containing 5-hydroxymethyl cytosine |
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Authors: | Hans van Ormondt Stanley Hattman |
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Affiliation: | 1. Department of Physiological Chemistry, University of Leiden, Sylvius Laboratories, Wassenaarseweg 72, Leiden, The Netherlands;2. the Department of Biology, University of Rochester, Rochester, New York 14627 USA |
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Abstract: | 32P-labeled oligonucleotides from a pancreatic DNase digest of non-glucosylated T2 gt? DNA have been analyzed by high voltage electrophoresis (both before and after dephosphorylation of the 5′ terminus). T2 gt? oligonucleotides, which contain 5-hydroxymethyl cytosine (hm5C) in place of cytosine (C), have altered electrophoretic mobilities compared to fd DNA oligonucleotides (which contain C). In addition, we have observed that pancreatic DNase exhibits a marked cleavage specificity; i.e., hm5C is the predominant 5′ terminal residue in the hm5C-containing oligonucleotides we have characterized. |
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