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NADH-cytochrome c reductase activity in cultured human lymphocytes: Similarity to the liver microsomal NADH-cytochrome b5 reductase and cytochrome b5 enzyme system |
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| Authors: | Russell A Prough Richard L Imblum Richard A Kouri |
| Institution: | 1. Department of Biochemistry and the UTHSC Cancer Center, The University of Texas Health Science Center at Dallas, 5323 Harry Hines Boulevard, Dallas, Texas 75235, USA;2. Department of Biochemical Oncology, Microbiological Associates, 4733 Bethesda Avenue, Bethesda, Maryland 20014 USA |
| Abstract: | A NADH-cytochrome c reductase activity was increased upon mitogen stimulation of human lymphocytes. The activity was not inhibited by antimycin A or rotenone but was specifically inhibited by antibodies elicited against rat liver NADH-cytochrome b5 reductase or cytochrome b5. The activity was linear with cellular homogenates up to 5.2 × 106 cells/ml and had abroad pH optimum of 7.7. The presence of 3-methylcholanthrene in mitogen stimulation media had no effect on the NADH-cytochrome c reductase activity but differentially induced the benzo(a)pyrene hydroxylase (AHH) activity. The reductase activity was present in nonstimulated cells and appears not to be significantly increased in activity per cell upon mitogen-stimulation of the peripheral lymphocyte. |
| Keywords: | To whom reprint requests should be sent |
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