A sensitive and reproducible procedure for the assay of arginyl-tRNA: Protein arginyl transferase |
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Authors: | Hiroo Horinishi Masatoshi Kato Toshio Takahashi |
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Institution: | Laboratory of Biological Chemistry, Food Research Institute of Morinaga & Co., Shimosueyoshi 2-1-1, Tsurumi-ku, Yokohama, Japan 230 |
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Abstract: | The activity of arginyl-tRNA: protein arginyl transferase was found to be enhanced four- to sevenfold by substituting bovine α-lactalbumin for bovine serum albumin, the standard acceptor protein used thus far. With α-lactalbumin as the acceptor protein in place of serum albumin, a sensitive and reproducible procedure for the transferase assay was established. |
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