Immobilization of hydrogenase on glass beads |
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| Authors: | Douglas A. Lappi Francis E. Stolzenbach Nathan O. Kaplan Martin D. Kamen |
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| Affiliation: | Department of Chemistry University of California, San Diego La Jolla, California 92093 USA;Chemical Biology Developmental Laboratory University of Southern California Los Angeles, California 90007 USA |
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| Abstract: | Hydrogenase from was immobilized on glass beads by four different methods. The sensitivity of the native and bound enzyme to oxygen was examined. Hydrogenase bound to succinyl glass proved to be the most stable to oxygen. All bound enzymes were active with ferredoxin as a substrate and evolved hydrogen in a chloroplast-ferredoxin-hydrogenase system driven by light. |
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