Staining and destaining polyacrylamide gels: A comparison of Coomassie blue and fast green protein dyes

A method using fast green dye for quantitation of immune serum globulin and its products of fragmentation in polyacrylamide gels has been developed. Although fast green is shown to be somewhat less sensitive than the usually used Coomassie blue stain, the former dye does not suffer from selective loss of dye due to temperature or alcohol content of the destaining solution. Destaining of fast green-stained gels is accomplished rapidly and efficiently by the described destaining procedure without an accompanying loss in quantitation.