A rapid and sensitive determination of bacterial rRNA by means of hybridization-competition |
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| Authors: | Ben A. Oostra Bert Zantinge Aly L. van Goor Albert J.J. van Ooyen Max Gruber |
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| Affiliation: | Biochemisch Laboratorium, Rijksuniversiteit, Zernikelaan, Groningen, The Netherlands |
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| Abstract: | A rapid and sensitive liquid hybridization-competition method for the assay of in vitro synthesized Escherichia coli rRNA was developed. Hybridization with homologous DNA and with heterologous DNA from Proteus vulgaris was compared. Besides unfractionated DNA we used DNA enriched for the sites complementary to ribosomal RNA by chromatography on methylated albumin Kieselguhr. Hybridization with heterologous enriched DNA is the method of choice due to its low background and high hybridization efficiency. |
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