Identification of the outer membrane protein of E.coli that produces transmembrane channels in reconstituted vesicle membranes |
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Authors: | Taiji Nakae |
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Institution: | Department of Bacteriology and Immunology University of California, Berkeley, California 94720 USA |
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Abstract: | Outer membrane of allows a rapid diffusion of saccharides of molecular weights less than 550. This permeability property could be restored in vesicle membranes reconstituted from isolated phospholipids, lipopolysaccharide, and an outer membrane protein. The active protein aggregates were isolated from the insoluble material left after solubilization of cell envelope of B with sodium dodecyl sulfate at 35°. Analysis by acrylamide gel electrophoresis, isoelectric focusing and amino terminal amino acid determination revealed that only a single species of protein, with a molecular weight of 36,500 forms the oligoprotein aggregates which produces diffusion channels. |
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Keywords: | SDS Sodium dodexyl sulfate LPS Lipopolysaccharide |
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