Improved method for removal of albumin from serum by affinity chromatography |
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Authors: | S. Aslam D.P. Jones T.R. Brown |
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Affiliation: | 1. Department of Medicine, Gastroenterology, and Pathology Hutzel Hospital Medical Unit Detroit, Michigan 48201 USA;2. Wayne State University School of Medicine Detroit, Michigan 48201 USA |
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Abstract: | Ges prepared from alkyl succinic anhydride coupled to agarose beads by diaminoalkane spacers have been studied to evaluate the influence of the chain length of both the alkyl succinic anhydride and the spacer on the gel's quantitative capacity and specificity to absorb albumin. The maximum absorptive eapacity for albumin of these gels varied from 13 to 30 mg of albumin/ml of gel and was related to the chain length of the alkyl succinic anhydride and the spacer. Before gel capacities were reached, eluates were albumin free when examined by electroimmunoassay (sensitivity, 1 μg/ml). The gels were not completely specific for albumin. However, pretreatment of the gels with gelatin decreased their nonspecific binding. |
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