What is the best method to remove 2,3-diphosphoglycerate from hemoglobin? |
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Authors: | Wolfgang Jelkmann Christian Bauer |
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Affiliation: | Physiologisches Institut der Universität, 84 Regensburg, West Germany |
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Abstract: | We have compared the efficiency of four methods to remove 2,3-diphosphoglycerate (DPG) from hemoglobin (Hb), comprising dialysis, repeated ultrafiltration, gel filtration, and ion-exchange chromatography. All the methods eventually yielded hemoglobin solutions with a ratio of <0.002 mol of DPG/mol of Hb4 and identical oxygen-binding properties but differed with respect to duration and versatility. Considering these factors, we found the combination of an ion-retardation resin and a mixed-bed resin to be most satisfactory. |
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