The molecular and catalytic properties of galactosyltransferase from fetal calf serum

A soluble galactosyltransferase (UDP-galactose:N-acetylglucosamine galactosyl-transferase) was purified to apparent homogeneity from fetal calf serum with an overall increase in specific activity of 19,600-fold. The enzyme exhibited the following properties: specific activity, 8.5 units/mg of protein; acceptor specificity, N-acetylglucosamine/ ovalbumin = 3.3; diffusion coefficient, 5.56; sedimentation coefficient, 3.2; and molecular weight, 47,800. Comparison of the structural and catalytic properties of the fetal calf serum enzyme with purified galactosyltransferase from bovine milk indicated that the enzymes from the two bovine sources are very similar and possibly identical.