Gas chromatographic determination of aromatic acid metabolites of phenylalanine in brain |
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| Authors: | Y.H. Loo L. Scotto M.G. Horning |
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| Affiliation: | 1. New York State Institute for Research in Mental Retardation, Staten Island, New York 10314 USA;2. Institute for Lipid Research, Baylor College of Medicine, Houston, Texas 77025 USA |
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| Abstract: | A method for the determination of the aromatic acid metabolites of phenylalanine in brain by gas-liquid chromatography is described. Procedures were developed for the extraction and purification of the metabolites, the preparation of their trimethylsilyl derivatives, the separation and identification of these derivatives by gas-liquid chromatography, and the quantification of the metabolites by employing the internal reference standards phenylvaleric and o-hydroxyphenylacetic acids with the detector molar response factors. The metabolites in the hyperphenylalaninemic brain were identified as the trimethylsilyl ester of phenylacetic, ester-ethers of mandelic and phenyllactic, and the ester-enol ether of the oxime of phenylpyruvic acid. |
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