Identification of a major extracellular non-collagenous glycoprotein synthesised by human skin fibroblasts in culture |
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Authors: | Christopher HJ Sear Michael E Grant David S Jackson |
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Institution: | Department of Medical Biochemistry, University of Manchester, Medical School, Oxford Road, Manchester M13 9PT, UK |
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Abstract: | The major protein released into the medium by human skin fibroblasts in culture has been shown to be a fucosylated glycoprotein (designated MFGP). Analysis by gel filtration chromatography and polyacrylamide gel electrophoresis demonstrated that under reducing conditions MFGP has a molecular weight of approx. 250,000, but occurs as a disulphide-linked aggregate in the medium. Three lines of evidence are presented to establish that MFGP is a non-collagenous molecule. |
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Keywords: | SDS sodium dodecyl sulfate |
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