Neurotransmitter release from viable purely cholinergic Torpedo synaptosomes |
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Authors: | Daniel M Michaelson Mordechai Sokolovsky |
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Institution: | The George S. Wise Center for Life Sciences Department of Biochemistry, Tel-Aviv University Tel-Aviv, Israel |
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Abstract: | Viable synaptosomes from the electric organ of Torpedo have been prepared and partially purified. The synaptosomes contain about 100 fold more acetylcholine (Ach) than do mammalian synaptosomes, synaptic vesicles and mitochondria. The Torpedo synaptosomes release Ach by K depolarization in the presence of Ca ions, and manifest an ionophore-mediated Ca-dependent Ach release. These results demonstrate that the synaptosomes contain the neurosecretion apparatus in a functional viable state. Since this preparation uniquely contains only one neurotransmission system (cholinergic), it is most suitable for structural and functional investigations of neuro transmission. |
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Keywords: | Ach Acetylcholine AchE Acetylcholinesterase EGTA Ethyleneglycol-bis-(β-Aminoethyl ether)N N′-Tetraacetic acid |
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