The determination of allopurinol and oxipurinol in human plasma and urine |
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Authors: | Martin Brown Alan Bye |
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Institution: | Department of Clinical Pharmacology, Wellcome Research Laboratories, Beckenham, Kent BR3 3BS Great Britain |
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Abstract: | A method is described for allopurinol and oxipurinol assay within human plasma and urine in the range expected during therapy. The method is based on high-performance ion-exchange chromatography following an efficient sample purification step using Chelex-100 resin in the Cu2+ form. Linear calibration curves are produced for allopurinol over the range 0.05–10 μmole/1 (0.068–1.36 μg/ml) in plasma and 0.005–1 mmole/1 (0.68–136 μg/ml) in urine and for oxipurinol 0.5–100 μmole/1 (0.076–15.2μg/ml) in plasma and 0.1–2 mmole/1 (15.2–304 μg/ml) in urine. |
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