Binding specificity of a HeLa DNA-binding protein to DNA and homopolymers |
| |
| Authors: | Takemi Enomoto Masa-atsu Yamada |
| |
| Institution: | Department of Physiological Chemistry, Faculty of Pharmaceutical Sciences, University of Tokyo Hongo, Tokyo 113, Japan |
| |
| Abstract: | A protein which has affinity for single-stranded DNA but not for double-stranded DNA has been isolated from HeLa cells by DNA-cellulose chromatography. This protein having a molecular weight of 34,000 was accounted for approximately 3% of total soluble proteins. Its binding specificity to DNA and nucleotide homopolymers has been investigated by Sephadex G-200 column chromatography. Specific binding to single-stranded DNA has been confirmed also by this method and furthermore strong binding to poly U has been found. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
