The role of fatty acid binding protein on the metabolism of fatty acids in isolated rat hepatocytes |
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Authors: | Maria YC Wu-Rideout Charles Elson Earl Shrago |
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Institution: | 1. Department of Nutritional Sciences, University of Wisconsin, Madison, Wisconsin 53706 USA;2. Department of Medicine, University of Wisconsin, Madison, Wisconsin 53706 USA |
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Abstract: | In isolated rat hepatocytes flavaspidic acid, a competitor with free fatty acids for the fatty-acid-binding-protein, decreased the uptake of oleic acid and triglyceride synthesis but stimulated the formation of CO2 and ketone bodies from oleic acid. Flavaspidic acid had no effect on the utilization of octanoic acid. Stimulation of the microsomal fatty-acid-activating enzyme by the fatty-acid-binding protein was reversed by flavaspidic acid. In contrast, the binding protein inhibited the mitochondrial fatty-acid-activating enzyme. Flavaspidic acid not only prevented this inhibition but actually stimulated the enzyme activity. The results indicate that the cytosol fatty-acid-binding protein directs the metabolism of long chain fatty acids toward esterification as well as enhancing their cellular uptake. |
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Keywords: | FFA free fatty acids FABP fatty-acid-binding protein |
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