Differential template specificities of nuclear RNA polymerases isolated from Physarum polycephalum |
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Authors: | Armin Hildebrandt Helmut W. Sauer |
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Affiliation: | Fachbereich Biologie der Universität Konstanz, Postfach 7733, 775 Konstanz, West Germany |
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Abstract: | RNA polymerases A and B from Physarum were more active on denatured homologous, calf thymus, or phage DNA than on the corresponding native templates. We obtained distinct patterns of template activities for various single- and double-stranded synthetic homopolymers and alternating copolymers. Some templates were copied asymmetrically. All dC-rich structures were highly active templates. Poly(dA) was efficiently transcribed only in combination with oligo(dT), not with poly(dT). Differential activities of enzymes A and B on several synthetic templates and phage DNA suggest different requirements for the RNA synthesis by the two RNA polymerases from Physarum. |
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