Double-label autoradiography on polyacrylamide gels with 3H and 14C

A method of autoradiography is deseribed which allows discrimination between ³H- and ¹⁴C-labeled materials on polyacrylamide gels. The method relies on a very simple procedure for switching from fluorography to autoradiography which results in a decrease in ¹⁴C detection sensitivity of about 10-fold but a concomitant decrease in ³H sensitivity of over 800-fold. With the proper ratio of ³H to ¹⁴C radioactivities, there is little or no “spillover” of one isotope during the detection of the other. No special equipment is required.