High-performance liquid chromatographic analysis of dianhydrogalactitol in plasma by derivatization with sodium diethyldithiocarbamate |
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| Authors: | Dennis Munger Larry A. Sternson Arnold J. Repta Takeru Higuchi |
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| Affiliation: | Department of Pharmaceutical Chemistry, McCollum Laboratories, University of Kansas, Lawrence, Kan. 66044 U.S.A. |
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| Abstract: | A high-performance liquid chromatographic method is described for measuring submicrogram quantities of dianhydrogalactitol, a promising anti-neoplastic agent, in plasma. The drug is derivatized directly in plasma with sodium diethyldithiocarbamate to form a bis(dithiocarbamoyl) ester which absorbs UV light at 254 nm (αm 17,000). The derivatized product is then extracted quantitatively into chloroform and separated by normal phase chromatography (μBondpak CN column). Dianhydrogalactitol concentration below 50 ng/ml of plasma can be detected in the eluent. |
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| Keywords: | To whom correspondence should be addressed |
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