Isoelectric focusing of brain adenylate cyclase |
| |
| Authors: | Douglas J Franks Daniel Malamud |
| |
| Institution: | 1. Shriners Burns Institute, Massachusetts General Hospital, Boston, Massachusetts 02114 USA;2. Department of Surgery, Harvard Medical School, Boston, Massachusetts 02115 USA |
| |
| Abstract: | Mouse brain adenylate cyclase has been solubilized with Lubrol PX and separated by isoelectric focusing on polyacrylamide gels. The enzyme activity has been measured with a sensitive assay isolating cyclic AMP from Dowex and alumina columns. The technique allows a one-step analysis of this membrane enzyme from a heterogeneous sample within 6 hr. |
| |
| Keywords: | All correspondence to Dr Malamud at the Shriners Burns Institute |
| 本文献已被 ScienceDirect 等数据库收录！ |
