Analysis of prednisolone in plasma by high-performance liquid chromatography |
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| Authors: | J.C.K. Loo A.G. Butterfield J. Moffatt N. Jordan |
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| Affiliation: | Pharmaceutical Chemistry Division, Drug Research Laboratories, Health Protection Branch, Ottawa, Ontario K1A 0L2 Canada |
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| Abstract: | A sensitive, specific high-performance liquid chromatographic procedure for the determination of prednisolone in plasma is described. The organic solvent extract from plasma is chromatographed on a silica gel column using a mobile phase of 0.2% glacial acetic acid, 6% ethanol, 30% methylene chloride in n-hexane on a high-performance liquid chromatograph fitted with an ultraviolet detector (254 nm). Quantitation of plasma samples containing 25 ng/ml prednisolone is reported. Metabolites and endogenous hydrocortisone do not interfere with prednisolone. The determination of prednisolone concentrations in plasma following administration of a 10-mg single oral dose to a human subject is described. |
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