Association of R6K plasmid replicative intermediates with the folded chromosome of |
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Authors: | M. Wlodarczyk B.C. Kline |
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Affiliation: | Department of Microbiology, Mayo Medical School, Mayo Foundation, Rochester, Minnesota 55901 USA |
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Abstract: | When strain CSH50(R6K) is lysed so as to preserve the folded chromosome structure approximately 9 of the 11 R6K molecules maintained per chromosomal equivalent cosediment with the host nucleoid on a neutral sucrose gradient; the remaining 2 plasmids sediment at their normal rate. When cells are briefly labeled with [3H]thymidine, the majority of plasmid replicative intermediates and nascent mature plasmids are found in the plasmid subpopulation that cosediments with host folded chromosomes. This finding suggests that plasmid replication occurs in a restricted cellular locus, perhaps even while in association with its host's folded chromosome. |
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