Lipid biosynthesis in developing and germinating soybean cotyledons: The formation of oleate by a soluble stearyl acyl carrier protein desaturase |
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Authors: | PK Stumpf RJ Porra |
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Institution: | Division of Plant Industry, Commonwealth Scientific and Industrial Research Organization, P.O. Box 1600, Canberra City, A.C.T.2601, Australia |
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Abstract: | Extracts of developing soybean cotyledons contain a highly specific stearyl acyl carrier protein (ACP) desaturase which in the presence of NADPH, O2, ferredoxin and ferredoxin: NADP+ reductase, rapidly converts stearyl ACP to oleyl ACP. The enzyme system has a high affinity for O2, near-maximal activity being obtained at only 10 μm O2. The pH optimum for the desaturase is 6.0. Stearic acid and stearyl-CoA, alone or in the presence of acyl carrier protein, are totally inactive. Although the enzyme is found in extracts prepared from developing soybean seeds (15–50 days after flowering), activity was not detected in extracts of germinated seeds. |
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